The ChromSword® Group

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ChromSword® AutoRobust - automated HPLC method robustness testing

ChromSword® AutoRobust is able to test the robustness of HPLC methods fully automatically to study the influence of variations in method parameters on chromatographic results.  The following parameters can be tested fully automatically:

  • flow rate

  • temperature

  • concentration of an organic modifier

  • gradient slope and break points positions

  • injection volume

  • equilibration time

  • column batches

  • buffer pH or concentration

  • wavelength

  • configuration of columns and solvents valves (if available) can be specified.

  • The basic, initial method to be tested for robustness can be specified or automatically imported from ReportViewer.

  • The absolute change in parameters to be tested, the number of steps to be performed for each parameter and a general plan (column batches, buffers, vial number) can be set up in seconds.

  • Parameters to be tested can be selected optionally for each experiment.

  • The ReportViewer supports robustness projects and reports the resolution for each pair, displays chromatograms, overlays them and reports the summary of robustness experiments.

ChromSword AutoRobust - HPLC method robustness testing

  • It also generates automatically a report as Microsoft Word documents with all methods, data and chromatograms.

  • Tables can be exported in Microsoft Excel and Microsoft Word. Back to the top

 

ChromSword® AutoRobust -
hard and software integration

  • Agilent 1100/1200/1200RR
    ChemStation    ® 16 bit versions A 09.01 and A 09.03 or higher
    ChemStation® 32 bit versions     B.01 and higher
    ChemStationPlus/Security pack compatible 
    EZChrom Elite® HPLC software (stand alone or client server)
      
    Binary/quaternary pumps, oven, auto-samplers,
    built in and external column and solvent switching valves,
    UV,     MWD, DAD, MSD, FLD, IR
    Light-Scattering Detector, Corona (requires Agilent A/D converter)

  • Hitachi LaChromElite®
    HSM , EZChromElite software
    (stand alone or client/server)

    Quarternary pump, autosampler, column oven with built-in
    3 positions column selection switching valve
    UV, DAD, FLD, IR

  • Waters Alliance
    Millennium 32, versions 3.2. and 4
    Empower and     Empower 2 Workstation
    (stand alone or client server)

    UV, PDA, W996 (DAD)
  • Knauer SmartLine LC system
    ChromGate software

    Quarternary pump, autosampler, column oven, UV, DAD, FLD
  • SWITCHING VALVES:
    • External Agilent 1100/1200
      6 positions column selector and
      12 positions solvent selector.
    • Waters
      6 positions column selector built in the Waters column oven (Waters Alliance system)
    • External VICI Valco
      2, 4, 6, 8, 10 positions column selectors and
      4-16 positions solvent selectors

ChromSword®AutoRobust - more information

pdf
ChromSword®AutoRobust presentation (pdf)

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