The ChromSword® Group

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CHIRIS™ - chiral columns

polysaccharide chiral stationary phases

Polysaccharide chiral stationary phases (CSPs) are recognized as the most useful CSPs for analytical and preparative purposes based on their broad chiral recognition abilities and high loading capacity  They are designed to cover a majority of chiral applications.

We offer three cellulose based CSPs and one amylose based CSP.

Cellulose based CSPs
CHIRIS-IOD
CHIRIS™ - IOD

   CHIRIS-IOB
CHIRIS™ - IOB		   CHIRIS-IOJ
CHIRIS™ - IOJ
   
     
Amylose based CSP
CHIRIS-IAD

CHIRIS™ - IAD

  
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high loadability - easy and cost effective upscaling

Cellulose based CSP Amylose CSP
CHIRIS-IOD
 5μm, 250 mm L x 4.6 mm ID
sample: trans-stilbene oxide
MP: hexane/2-propanol 90:10
UV detection: 254 nm
flow rate: 1.0 mL/min		 CHIRIS-IAD
 5μm, 250 mm L x 4.6 mm ID
sample: trans-stilbene oxide
MP: : hexane/2-propanol 90:10
UV detection: 254 nm
flow rate: 1.0 mL/min
   
Chiris-IOD loadability Chiris-IAD loadability
21 mg, 15 mg, 2.5 mg, 0.5 mg, 50 µg 30 mg, 15 mg, 2.5 mg, 0.5 mg, 50 µg
   


reproducibility test

Cellulose based CSP Amylose CSP
CHIRIS-IOD
 5μm, 250 mm L x 4.6 mm ID
sample: trans-stilbene oxide +
1,3,5-tri-tert-butylbenzene
MP: hexane/2-propanol 90:10
UV detection: 254 nm
flow rate: 1.0 mL/min
conditioning:  30 min. prior injection
of 80 µg sample		 CHIRIS-IAD
 5μm, 250 mm L x 4.6 mm ID
sample: trans-stilbene oxide +
1,3,5-tri-tert-butylbenzene
MP: : hexane/2-propanol 90:10
UV detection: 254 nm
flow rate: 1.0 mL/min
conditioning:  30 min. prior injection
of 80 µg sample
   
Chiris-IOD reproducibility  Chris-IAD reproducibility 
                           
#  tR1 tR2 a Rs  N1 N2 #  tR1 tR2 a  Rs N1 N2
1  5.517  8.475  2.10  3.89  7264  5894  1  5.067  9.300  2.85  7.70  10081  8551 
2  5.517  8.458  2.09  3.84  7012  5591  2  5.075  9.292  2.85  7.81  10496  9026 
3  5.492  8.433  2.10  3.82  6826  5645  3  5.058  9.275  2.86  7.96  10447  8846 
                           
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selected applications

CHIRIS-IOD   5μm, 250 mm L x 4.6 mm ID, MP: hexane/2-propanol 90:10, UV detection: 254 nm, flow rate: 1.0 mL/min, pressure:  2.0 MPa
 Chiris-IOD application2
  Chiris-IOD application3 Chiris-IOD application1 Chiris-IOD application4
CHIRIS-IOJ   5μm, 250 mm L x 4.6 mm ID, MP: hexane/2-propanol 90:10, UV detection: 254 nm, flow rate: 1.0 mL/min, pressure:  2.1 MPa 
   
Chiris-IOJ application2  Chiris-IOJ application3  Chiris-IOJ application1 Chiris-IOJ application 4
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ordering information

Please enter IOD, IOB, IOJ or IAD in front of the order number, e.g.

  • IOD-5-150-4.6     (Chiris-IOD, 5 µm, 150 x 4.6 mm) or  
  • IAD-20-250-20     (Chiris-IAD, 20 µm, 250 x 20 mm)
Column
(length x ID, mm)		 5 µm 10 µm 20 µm
150 x 4.6 5-150-4.6 10-150-4.6 20-150-4.6
250 x 4.6 5-250-4.6 10-250-4.6 20-250-4.6
30 x 8 5-30-8 10-30-8 20-30-8
150 x 8 5-150-8 10-150-8 20-150-8
250 x 8 5-250-8 10-250-8 20-250-8
30 x 16 5-30-16 10-30-16 20-30-16
150 x 16 5-150-16 10-150-16 20-150-16
250 x 16 5-250-16 10-250-16 20-250-16
30 x 20 5-30-20 10-30-20 20-30-20
150 x 20 5-150-20 10-150-20 20-150-20
250 x 20 5-250-20 10-250-20 20-250-20
30 x 32 5-30-32 10-30-32 20-30-32
150 x 32 5-150-32 10-150-32 20-150-32
250 x 32 5-250-32 10-250-32 20-250-32
150 x 40 5-150-40 10-150-40 20-150-40
250 x 40 5-250-40 10-250-40 20-250-40
300 x 62 5-300-62 10-300-62 20-300-62
           
Further dimensions are available upon request.

Bulk material is available in 10 µm and 20 µm. 
Please inquire at info@iristech.net.


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"brush type" (or "Pirkle-type") chiral phases

CHIRIS™-IB phases are "brush-type" (or "Pirkle-type") Chiral Stationary Phases (CSPs). 
They contain a small chiral selector covalently bound to the silica surface via a spacer. 

Generally, they fall into three categories:

  • p-electron acceptor / p-electron donors,

  • the p-electron acceptors and

  • the p-electron donors.

With this "brush-type" CSPs chiral recognition occurs at binding sites; p-basic or p-acidic aromatic rings, acidic sites, basic sites, and through steric interactions. 

"brush type" phases CHIRIS™IB-1, IB-2, IB-3, IB-4, IB-5, IB-6
CHIRIS-IB1 structure

CHIRIS™-IB-1
(formerly known as CHIRIS-AD2)

  

 

 

CHIRIS-IB2 structure

 

 

 

CHIRIS™-IB-2
 
 
CHIRIS-IB3 structure



CHIRIS™-IB-3		   CHIRIS-IB4 structure

CHIRIS™-IB-4
 
     
CHIRIS-IB5 structure

CHIRIS™-IB-5
(formerly known as CHIRIS-PBI)		   CHIRIS-IB6 structure
CHIRIS™-IB-6
 

 

selected applications

Back to the top  CHIRIS-IB-1

hexane/dichloromethane/methanol (100:30:1), 2 mL/min, 254 nm

hexane/dichloromethane/methanol (100:30:1), 1 mL/min, 254 nm 
CHIRIS-IB-1, application 1  CHIRIS-IB-1, application 2  CHIRIS-IB-1, application 3   CHIRIS-IB-1, application 4
Back to the top  CHIRIS-IB-2      
hexane/2-propanol (70:30),
+ 0.1% trifluoroacetic acid,
+ 0.1% diisopropylamine)
1.0 mL/min, 225 nm		 hexane/2-propanol (90:10),
+ 0.1% trifluoroacetic acid,
+ 0.1% diisopropylamine)
1.0 mL/min., 254 nm		 hexane/2-propanol (70:30),
+ 0.1% trifluoroacetic acid,
+ 0.1% diisopropylamine)
1.0 mL/min, 225 nm		 hexane/2-propanol (90:10),
+ 0.5% acetic acid
1.0 mL/min., 254 nm
CHIRIS-IB-2 application 1 CHIRIS-IB-2 application 2 CHIRIS-IB-2 application 3 CHIRIS-IB-2 application 4
hexane/2-propanol (90:10)
+ 0.5% acetic acid
1.0 mL/min., 254 nm		 hexane/dichloromethane/methanol  (100:30:1),
2.0 mL/min., 254 nm		    
CHIRIS-IB2 application 5 CHIRIS-IB2 application 6    
Back to the top  CHIRIS-IB-3      
hexane/dichloromethane/methanol  (100:30:1),
1.0 mL/min., 254 nm		 methyl-t-butylether/methanol (90:10)
2.0 mL/min, 254 nm		 hexane/dichloromethane/methanol  (100:30:1),
1.0 mL/min., 254 nm
CHIRIS-IB-3 application 1 CHIRIS-IB-3 application 2 CHIRIS-IB-3 application 3 CHIRIS-IB-3 application 4
hexane/dichloromethane/methanol  (100:30:1),
1.0 mL/min., 254 nm		      
CHIRIS-IB-3 application 5      
Back to the top  CHIRIS-IB-4      
hexane/2-propanol (90:10), 1.0 mL/min., 254 nm    
CHIRIS-IB-4 application 1 CHIRIS-IB-4 application 2 CHIRIS-IB-4 application 3 CHIRIS-IB-4 application 4
hexane/2-propanol (90:10),
2.0 mL/min., UV (254 nm) and CD 		hexane/2-propanol (90:10)
2.0 mL/min., 254 nm		    
CHIRIS-IB-4 application 5 CHIRIS-IB-4 application 6    
Back to the top  CHIRIS-IB-5      
hexane/2-propanol (90:10), 1.0 mL/min., 235 nm hexane/2-propanol (90:10) + 0.5% acetic acid, 1.0 mL/min., 235 nm
CHIRIS-IB-5 application 1 CHIRIS-IB-5 application 2 CHIRIS-IB-5 application 3 CHIRIS-IB-5 application 4
Back to the top  CHIRIS-IB-6      
hexane/2-propanol (90:10)
+ 0.5% acetic acid
2.0 mL/min., 254 nm		 hexane/2-propanol (80:20)
1.0 mL/min., 254 nm		 hexane/2-propanol (80:20)
2.0 mL/min., 254 nm		 hexane/2-propanol (90:10)
1.0 mL/min., 254 nm
CHIRIS-IB-6 application 1 CHIRIS-IB-6 application 2 CHIRIS-IB-6 application 6 CHIRIS-IB-6 application 4
       
 

Back to the top  ordering information

Please enter IB1, IB2, IB3, IB4, IB-, or IB6 in front of the order number, e.g.

  • IB1-10-250-8     (Chiris-IB-1, 10 µm, 250 x 8 mm) or
  • IB3-5-150-4.6    (Chiris-IB-3, 5 µm, 150 x 4.6 mm)
     
Column
(length x ID, mm)		 5 µm 10 µm
150 x 4.6 5-150-4.6 10-150-4.6
250 x 4.6 5-250-4.6 10-250-4.6
30 x 8 5-30-8 10-30-8
150 x 8 5-150-8 10-150-8
250 x 8 5-250-8 10-250-8
30 x 16 5-30-16 10-30-16
150 x 16 5-150-16 10-150-16
250 x 16 5-250-16 10-250-16
30 x 20 5-30-20 10-30-20
150 x 20 5-150-20 10-150-20
250 x 20 5-250-20 10-250-20
30 x 32 5-30-32 10-30-32
150 x 32 5-150-32 10-150-32
250 x 32 5-250-32 10-250-32
150 x 40 5-150-40 10-150-40
250 x 40 5-250-40 10-250-40
300 x 62 5-300-62 10-300-62
         
Further dimensions are available upon request.
Please inquire at info@iristech.net.
 


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